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Only 2 of Klebsiella isolates 0.

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Martinsa Adriana G. Open in a separate window.

These data might suggest that, although genetically related, the isolates were probably subjected to distinct selective pressures, which could have resulted in different OMP modifications. BKC-1 Brazilian Klebsiella carbapenemase is a class A serine carbapenemase that was recently identified in 504 pneumoniae isolates from Brazil that belonged to sequence type ST clonal complex [CC] 2.

Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically; approved standard— 10th ed CLSI document MA BKC-1 is a new class A serine carbapenemase that was recently identified in Klebsiella pneumoniae clinical jcn. Performance standards for antimicrobial susceptibility testing; 25th informational supplement.

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To determine whether this jc was identical to p, the first plasmid identified as carrying bla BKC-1we performed whole-plasmid sequencing of the plasmid recovered from KpnJ. To date, inactivation of mgrB has been the main mechanism of polymyxin resistance observed in K. Support Center Support Center. Frequency of BKCproducing Klebsiella species isolates.

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Published online Jul The Kpn and KpnJ strains showed resistance to all beta-lactams and fluoroquinolones. The other authors have no conflicts to declare.

Author information Article notes Copyright and License information Disclaimer. Address correspondence to Willames M. The principal objective of this study was to evaluate the frequency of bla BKC-1 by testing a jmc of Klebsiella isolates. Antimicrobial susceptibility profiles of BKCproducing K.

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Prepublished online May The carbapenemase was encoded by bla BKC-1which was located on a small nonconjugative plasmid 50466. Nicolettia Silvia R.

Please review our privacy policy. Diagn Microbiol Infect Dis The mgrB icm as a key target for acquired resistance to colistin in Klebsiella pneumoniae. The bla BKC-1 gene was inserted into a kb plasmid that was identical to the previously reported plasmid, p National Center for Biotechnology InformationU.

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Santosb Jorge L. Sampaiob, c and Ana C.

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In the past decade, carbapenemase-encoding genes such as bla KPC-likebla OXAand bla NDM-like have emerged as the main mechanisms of carbapenem resistance in Enterobacteriaceae.

Plasmid extraction of K.

The Klebsiella strains cjm isolated from different clinical specimens obtained between and Fig. A Map of Brazil, showing the geographic distribution of Klebsiella isolates that were investigated for the bla BKC-1 gene.

To evaluate the stability of p harbored by the Kpn isolate, we performed serial passages of the Kpn isolate on Mueller-Hinton agar plates without the addition of antibiotic.

We verified that, in the absence of selective pressure, the plasmid harboring the bla BKC-1 gene, p, was unstable.