JCM 5046 PDF

14 products offers 14 jcm ic products. About 28% of these are integrated circuits. A wide variety of jcm ic options are available to you. JCM datasheet, JCM datasheets and manuals electornic semiconductor part. VI- J2JCM, VI-J3JCM, VI-J4JCM, VI-J5JCM and other pdf’s. Record 1 – 15 of 15 Product Detail: Offer JCM SANYO, TS12TCC, LMHNOPB from Hong Kong Inventory.

Author: Fesar Dakasa
Country: Spain
Language: English (Spanish)
Genre: Photos
Published (Last): 11 February 2012
Pages: 312
PDF File Size: 18.69 Mb
ePub File Size: 12.91 Mb
ISBN: 769-5-71387-478-2
Downloads: 21905
Price: Free* [*Free Regsitration Required]
Uploader: Kajishicage

Performance standards for antimicrobial susceptibility testing; 25th informational supplement. To evaluate the stability of p harbored by the Kpn isolate, we performed serial passages of the Kpn isolate on Mueller-Hinton agar plates without the addition of antibiotic. However, Southern blot analysis revealed that the bla BKC-1 gene was located on the same kb plasmid in 50046 isolates. Sampaiob, c and Ana C.

BKC-1 is a new class A serine carbapenemase that was recently identified in Klebsiella pneumoniae clinical isolates. The other authors have no conflicts to declare. Antimicrobial susceptibility profiles of BKCproducing K.

JCM5045N, JCM5046, JCM5046N

Author information Article notes Copyright and License information Disclaimer. Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically; approved standard— 10th ed CLSI document MA Kpn showed susceptibility to tigecycline MIC, 0.

S, sensitive; R, resistant. National Center for Biotechnology InformationU. This article has been cited by other articles in PMC.

Support Center Jcmm Center. Antimicrob Agents Jfm Address correspondence to Willames M. A total of Klebsiella strains collected from previous surveillance studies were randomly selected for this study, in order to represent distinct Brazilian geographic regions.


These data might suggest that, although genetically related, the isolates were probably subjected to distinct selective pressures, which could have resulted in different OMP modifications. Nicolettia Silvia R. In this ucm, a low frequency of isolates carrying bla BKC-1 was observed and was mainly attributed to the persistence of a K. Prepublished online May Published online Jul It is important to note that such new studies must search for bla BKC-1 using molecular techniques, since phenotypic tests may not accurately detect BKCproducing isolates 8.

The 50466 was encoded by bla BKC-1which was located on a small nonconjugative plasmid 2. During the experiments, it was observed that bla BKC-1 was easily lost in the absence of selective pressure. A Map of Brazil, showing the geographic distribution of Klebsiella isolates that were investigated for the bla BKC-1 gene.

Carbapenemase-producing Klebsiella pneumoniae, a key pathogen set for global nosocomial dominance.

Comparison of phenotypic tests for detecting BKCproducing Enterobacteriaceae isolates. J Clin Microbiol Initially, we performed transformation by electroporation into Escherichia coli Top BKC-1 Brazilian Klebsiella carbapenemase is a class A serine 504 that was recently identified jck Klebsiella pneumoniae isolates from Brazil that belonged to sequence type ST clonal complex [CC] 2. To determine whether this plasmid was identical to p, the first plasmid identified as carrying bla BKC-1we performed whole-plasmid sequencing of the plasmid recovered from KpnJ.

In the past decade, carbapenemase-encoding genes such as bla KPC-likebla OXAand bla NDM-like have emerged as the main mechanisms of carbapenem resistance in Enterobacteriaceae. In conclusion, prudent use of antimicrobials and strict adherence to infection control measures seem to be the only effective tools for preventing the acquisition and spread of new carbapenemase-encoding genes.


Martinsa Adriana G. Clinical jm Laboratory Standards Institute. Transformant cells were selected mcm LB agar plates supplemented with 0.

Frequency of BKCProducing Klebsiella Species Isolates

The Kpn and KpnJ strains showed different plasmid profiles. In addition, bla BKC-1 was harbored by a small unstable plasmid that could be easily lost in the absence of selective pressure. Please 5406 our privacy policy. Only 2 BKCproducing K. More studies with greater numbers of isolates from all Brazilian states are necessary to assess the real prevalence of isolates carrying bla BKC Multilocus sequence typing of Klebsiella pneumoniae nosocomial isolates.

J Antimicrob Chemother Antimicrobial susceptibility testing was performed by the broth microdilution method, according to CLSI guidelines 45.

Only 2 of Klebsiella isolates 0. We verified that, in the absence of selective pressure, the plasmid harboring the bla BKC-1 gene, p, was unstable. Diagn Microbiol Infect Dis Since BKC-1 is a weak carbapenemase and other mechanisms could be associated with carbapenemase production, we selected Klebsiella strains independent of the resistance phenotype.

Santosb Jorge L. The mgrB gene as a key target for acquired resistance to colistin in Klebsiella pneumoniae.

Plasmid extraction of K. The Kpn and KpnJ strains showed resistance to all beta-lactams and fluoroquinolones. Abstract BKC-1 is a new class A serine carbapenemase that was recently identified in Klebsiella pneumoniae clinical isolates. The principal objective of this study was to evaluate the frequency of bla BKC-1 by testing a collection of Klebsiella isolates. Open in a separate window.